Molecular Characterization of the Croatian Autochthonous Istrian Cattle

Jelena Prpić, Tomislav Keros, Lorena Jemeršić

Abstract


The purpose of the study was to improve knowledge on the genetic structure status of the Croatian autochthonous Istrian cattle, breed currently listed by the FAO as “critically endangered”. The study included 20 randomly selected cattle blood samples on which genotyping was performed using 11 microsatellites from the panel recommended by the ISAG / FAO. The procedure enabled the identification of as many as 209 (94.14%) genotypes and, at the same time 114 allelic variations were found with = 10.36 alleles per locus. The genotypes showed differences between the microsatellite loci and also significant differences in the frequency of both homozygosity and heterozygosity. The analysis of the occurrence of the microsatellite loci showed marked allelic variability. The distribution of genetic relationship was expressed by F coefficients, and a moderate level of genetic diversity was observed at some loci. The findings demonstrate the degree of breed relationship and breed purity, but also indicate threats to certain parts of the genome of the studied Istrian cattle. Therefore, urgent measures must be taken to support both the genetic consolidation and preservation of the remaining population of “purebred” samples.

Keywords


Genetic diversity; Genetic structure; Istrian cattle; Microsatellites; Podolian cattle

Full Text:

PDF

References


Belkhir, K., Borsa, P., Goudet, J., Chikhi, L., & Bonhomme, F. (2002). F- Genetix 4.0 logical sous windows TM pour la genetique des populations. Laboratorie Genome populations, interactions, CNRS upr 9060. Univ Montpellier II. Avaliable from: http//www.univ-montpl.fr/htm

Bruford, M. W., Bradley, D. C., & Luikart, G. (2003). DNA markers reveal the complexity of livestock domestication. Nature Reviews Genetics 4, 900-909.

Croatian Agricultural Agency 2017. Annual report for 2016.-Cattle breeding. CAA, Zagreb.

D,Andrea, M., Pariset, L., Matassino, D., Valentini, A., Lenstra, J.A., Maiorano, G., &Pilla, D. (2011). Genetic characterisation and structure of the Italian Podolian cattle breed and its relationship with some major European breeds. Italian Journal of Animal Science, 10, 237-243.

Georgescu, S.E., Manea, M.A., Zeulet, M., & Costache, M. (2009). Genetic diversity among Romanian cattle breeds with a special focus on the Romanian grey steppe breed. Romanian Biotechnological Letters, 14, 4194-4200.

Gibson, J.P., Ayalew, W., &Hanotte, O. (2007). Measures of diversity as inputs for decision in conservation of liverstock genetic resources, in: Javis, S.L., Padock, C., Cooper, H.D. (Eds.), Managing biodiversity in agricultural ecosystems. Columbia University Press, New York, US, 117-140.

ISAG/FAO, Standing Committe Recomandations, 2004. Available from: http:// dad.fao.org/cgi-bn/getblob.egi2sid=1.500006220

Ivanković, A., Orbanić, S., Caput,P., Mijić, P., Konjačić, V., &Bulić, V. (2006a). The genetic structure and sustainability of autochthonous cattle breeds in Croatia. Stočarstvo, 60, 47-51.

Ivanković, A., Orbanić, S., Caput, P., Mijić, P., Konjačić, V., Ramljak, J., Špehar, M., &Bulić, V. (2006b). Inventory in Croatia. Stočarstvo, 60, 243-257.

Ivanković, A., Kelava, N., Konjačić, M., Caput, P., & Ramljak, J. (2006c9. Exterior features of Istrian cattle. Stočarstvo, 61, 161-173.

Diouf, J. (2007). Global plan of action for animal genetic resources and Interlaken declaration. FAO, Rome.

Kaps, M., &Lamberson, W. R. (2004). Biostatistics for animal science. CABI. Wallingford, Cambridge.

Keros, T., Jemeršić, L., Prpić, J., &Brnić, D. (2015). Molecular characterization of autochthonous Slavonian Syrmian Podolian cattle. Acta Veterinaria Beograd, 65, 89-98.

Kovač, M. (1990). Istrian cattle, in: Štifanić, A., Kovač, M. (Eds.), Istrian cattle a Monograph Višnjan. Association of Istrian cattle breeders – SUIL, 24-29.

Maretto, F., Ramljak, J., Sbgrra, F., Penasa, M., Mantovani, R., Ivanković, A., & Bittunte, G. (2012). Genetic relationship among Italian and Croatian Podolian cattle breeds assessed by microsatellite maners. Livestock Science, 150, 256-264.

Posavi, M., Ozimec, R., Ernoić, M., & Poljak, F. (2003). Encyclopaedia of Croatian Domestic Animals. Varaždin, Katarina Zrinski, 51 - 53.

Ramljak, J., Ivanković, A., Veit-Kensch, C. E., Förster, M., & Međugorac, J. (2011). Analysis of genetic and cultural conservation value of three indigenous Croatian cattle breeds in local and global context. Animal Breeding & Genetics, 128, 73-84.

Schaefer, B. D. (20039. World Watch list for domestic animal diversity. 1 Ed. FAO, Rome.

Teneva, A., Todorovska, E., Tynfekchiev, N., Stela, A., Boettcher, P., & Dimitrov, L. (2007). Molecular characterisation of Bulgarian genetic resources: II. Microsatellite variation within and among Bulgarian cattle breeds. Biotechnology in Animal Husbandry, 23, 227-242.


Refbacks

  • There are currently no refbacks.


Copyright © 2018 Scholars Journal of Research in Agriculture and BiologyAll rights reserved.

ISSN: 2456-6527

For any query/support contact us at sjrabeditor@scischolars.com, agribiojournal@gmail.com,

editorial@scischolars.com