Fast and Efficient DNA Extraction Method for a Plumeria acuminata with Medicinal Potential

Ashwini A. Waoo, Sana Tarannum

Abstract


Many herbs are used from ancient to cure diseases. Plumeria species is of Apocynaceae family, Plumeria accuminata belonging to this, is also considered as ornamental plant. The plant is full of medicinal uses as it cures infections, digestive diseases, bronchitis, bleeding piles, dysentery, blood disorder, blennorrhagia, leprocy, rheumatism. The biological activities are anti-inflammatory, wound healing activity, anti-pyretic action, anti-tumor potential and antioxidant properties, antifertility, antimicrobial, hepatoprotectant activities.

A simple, rapid and efficient method developed for the extraction of genomic DNA from Plumeria accuminata leaves. The protocol was based on a modified cetyl trimethylammonium bromide (CTAB) extraction method, allowed the rapid DNA extraction from little amounts of leaf material. This protocol gives good quality DNA, yield and a shorter duration of extraction would be ideal for genotyping and further studies.

Keywords


DNA; CTAB; Genotyping, Medicinal uses; Plumeria acuminate.

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References


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