An Efficient Callus Induction Protocol for Gyrinops walla Gaetner ‘Walla patta’, a commercially important Agarwood species in Sri Lanka

S. P Munasinghe, S. R. Weerakoon, S. Somaratne, C. Ranasinghe

Abstract


Gyrinops walla, an endemic to Sri Lanka, is the only plant known to produce Agarwood, a resinous substance used in perfume industries. Increasing global demand on Agarwood leads the over-exploitation of G. walla to the verge of extinction. Raising callus facilitate sustainable utilization of G. walla avoiding destructive harvesting.  The objective of the present study was to raise callus from the explants of G. walla. The tender leaves and nodes were surface sterilized in 70% alcohol and 0.2% (w/V) HgCl2, aseptically. Ex-plants were cultured in modified MS medium supplemented with 3% (w/V) sucrose and gelled with 1% (w/V) agar with varying hormonal concentrations. Following 60 days incubation, weight of the callus was measured and callus induction percentage was calculated.  Initiation of callus was observed in all the hormones combinations and the highest weight and callus induction percentage were observed for leaves and nodal explants in  MS medium supplemented with BAP (0.5 mg/l) + NAA (3mg/l) and BAP (0.5 mg/l) + NAA (1.5 mg/l), respectively, yielding the highest fresh weight and callus induction.

 

 

 


Keywords


Gyrinops walla Gaetner., BAP, NAA, MS

Full Text:

PDF

References


Abdin, J. (2014). The Bangladeshi Agarwood Industry: Development Barriers and a Potential Way Forward. International Journal of Economics & Management Sciences 3(1):

Akter, A., and A. Neelim (2008). Agarwood Plantation at BRAC Tea Estate: Introduction, Environmental Factors and Financial Analysis. BRAC Research Report, Research and Evaluation Division BRAC Centre, 75 Mohakhali, Dhaka 1212, Bangladesh.

Chakrabarty, K., Kumar, A., and Menon, V. (1994). Trade in Agarwood. TRAFFIC India and WWF- India, New Delhi. 51 pp.

CITES – Convention on International Trade in Endangered Species of Wild Fauna and Flora (2005). Notification to the Parties No. 2005/014. (www document).

Compton, J.G.S., Zich, F.A. (2002) Gyrinops ledermannii (Thymelaeaceae), being an agarwood-producing species prompts call for further examination of taxonomic implications in the generic delimitation between Aquilaria and Gyrinops. Flora Malesiana Bullatin 13(1): 61–65.

Crous, P. W., Gams, W, Wingfield M. J., and P. S. van Wyk (1996).Phaeoacremoniumgen. nov.associatedwith wilt and decline diseases of woody hosts and human infections, Mycologia 88(5): p. 786–796.

Daud, N. H., ShashitaJayaraman , S. and R. Mohamed (2012) An improved surface sterilization technique for introducing leaf, nodal and seed explants of Aquilariamalaccensisfrom field sources into tissue culture. As. Pac. J. Mol. Biol. Biotechnol. 20 (2), 55-58.

Dharmadasa, R.M., A. Siriwardana, K. Samarasinghe, and P. Adhihetty (2013) Standardization of Gyrinops Walla Gaertn. (Thymalaeaceae): Newly Discovered, Fragrant Industrial Potential, Endemic Plant from Sri Lanka. World Journal of Agricultural Research 1(6) :101-103.

Donovan, D. and Puri, R. (2004). Learning from traditional knowledge of non-timber forest products: Penan Benalui and the autecology of Aquilaria in Indonesian Borneo. Ecology and Society 9(3): 3. [online] URL: http://www.ecologyandsociety.org/vol9/iss3/art3/

Fratkin J (1994).Chinese Herbal Patent Formulas: A Practical Guide.Shyaublications, Colorado, USA. 356pp.

Gaspar, T., Kevers, C., Penel, C., Greppin, H., Reid, D.M., Thorpe, T.A. (1996). Plant Hormones and Plant Growth Regulators in Plant Tissue Culture. In Vitro Cellular and Developmental Biology - Plant 32:272-289.

Liu, Y., Chen, H., Yang, Y., Zhang, Z., Wei, J., Meng, H., Chen, W., Feng, J., Gan, B., Chen, X., Gao, Z., Huang, J., Chen, B., and Chen, H. (2013). Whole-tree Agarwood-Inducing Technique: An Efficient Novel Technique for producing High-Quality Agarwood in cultivated Aquilaria sinensis Trees. Molecules 18(3): 3086-3106.

Meng-ling, M., Shu-yuan, Q., Lan-juan, H. (2005). Rapid in vitro propagation of medicinally important Aquilaria agallocha. Journal of Zhejiang University Science, 6:849-852

MOE, 2012. The Notational Red List 2012 of Sri Lanka. Conservation status of the Fauna and Flora. Ministry of Environment, Colombo, Sri Lanka, Viii + 476 pp.

Naef, R. (2011). The volatile and semi- volatile constituents of agarwood, the infected heartwood of Aquilaria species: A review. Favour Fragr. J. 26: 73-89.

Okudera, Y., and M. Ito (2009 ) Production of agarwood fragrant constituents inAquilariacalliand cell suspension cultures. Plant Biotechnology 26, 307–315.

Okugawa, H., Ueda, R., Matsumoto, K., Kawanishi, K., and Kato, K. (2000). Effects of sesquiterpenoids from "Oriental incenses" on acetic acid-induced writhing and D2 and 5-HT2A receptors in rat brain. Phytomedicine, 7, 417 422.

Takemoto H, Ito M, Shiraki T, Yagura T, Honda G (2008). Sedative effects of vapour inhalation of agarwood oil and spikenard extract and identification of their active components. J. Nat. Med., 62: 41-46.

Townsend, C. C. (1981). Thymeliaceae. In A revised Handbook to the Flora of Ceylon, Vol. II, M. d. Dassanyake and F. R. Fosberg (edts.), Amerind Publishing Co. Pvt. Ltd., New Delhi.

Van Beek, H. H., and D. Phillips. (1999) Agarwood: Trade and CITES implementation in Southeast Asia, Unpublished report prepared for TRAFFIC Southeast Asia, Malaysia.

Van Minh, T. (2005). Application of Tissue Culture Techniques in Woody species Conservation, Improvement in Vietnam: Agarwood (Aquilariacrassna Pierre ex LeComte) via Shoot tip culture. Proc. 2nd IS On Biotech. Of Trop. and Subtrop. Species, Acta Hort. 692, W. C. Chang and R. Drew (eds, pp.37-42.

Yang JS, Wanf YL, Su YL, He DH, Zheng QT, Yang J (1989). Studies on the chemical constituents of Aquilaria sinensi s(Luor) Gilg. Elucidation of the structure of the isobaimuxinol and isolation and identification of the constituents of lower boiling fraction of the volatile oil. Yao Xue Xue Bao, 24(4): 264-268.


Refbacks

  • There are currently no refbacks.


Copyright © 2018 Scholars Journal of Research in Agriculture and Biology. All rights reserved.

ISSN: 2456-6527

For any query/support contact us at sjrabeditor@scischolars.com, ssroscischolars@gmail.com